ARG45560
anti-PLCD3 antibody
anti-PLCD3 antibody for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PLCD3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PLCD3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human PLCD3. |
| Conjugation | Un-conjugated |
| Alternate Names | PLCD3; Phospholipase C Delta 3; PLCD3; Phospholipase C Delta 3; 1-Phosphatidylinositol 4,5-Bisphosphate Phosphodiesterase Delta-3; Phosphoinositide Phospholipase C-Delta-3; PLC-Delta-3; EC 3.1.4.11; 1-Phosphatidylinositol-4,5-Bisphosphate Phosphodiesterase Delta-3; Phospholipase C, Delta 3; Phospholipase C-Delta-3; PLC Delta3; KIAA1964 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 94 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q8N3E9 Human 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase delta-3 |
|---|---|
| Gene Symbol | PLCD3 |
| Gene Full Name | Phospholipase C Delta 3 |
| Background | This gene encodes a member of the phospholipase C family, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate to generate the second messengers diacylglycerol and inositol 1,4,5-trisphosphate (IP3). Diacylglycerol and IP3 mediate a variety of cellular responses to extracellular stimuli by inducing protein kinase C and increasing cytosolic Ca(2+) concentrations. This enzyme localizes to the plasma membrane and requires calcium for activation. Its activity is inhibited by spermine, sphingosine, and several phospholipids. [provided by RefSeq, Jul 2008] |
| Function | Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores. Essential for trophoblast and placental development. May participate in cytokinesis by hydrolyzing PIP2 at the cleavage furrow. [UniProt] |
| Cellular Localization | Cytoplasm, Membrane. [UniProt] |
| Calculated MW | 89 kDa |
| PTM | Phosphoprotein. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG45560 anti-PLCD3 antibody ICC/IF image
Immunofluorescence: CACO-2 stained with ARG45560 anti-PLCD3 antibody at 5 μg/ml dilution.
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ARG45560 anti-PLCD3 antibody WB image
Western blot: RT-4 stained with ARG45560 anti-PLCD3 antibody at 0.5 μg/ml dilution.
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ARG45560 anti-PLCD3 antibody FACS image
Flow Cytometry: HL-60 stained with ARG45560 anti-PLCD3 antibody at 1 µg/10^6 cells dilution.
