ARG43614
anti-PLAP / Placental alkaline phosphatase antibody
anti-PLAP / Placental alkaline phosphatase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PLAP / Placental alkaline phosphatase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PLAP / Placental alkaline phosphatase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a.a. 403-433 of Human PLAP / Placental alkaline phosphatase. |
| Conjugation | Un-conjugated |
| Protein Full Name | Alkaline phosphatase, placental type |
| Alternate Names | PALP; PLAP; EC 3.1.3.1; Alkaline phosphatase Regan isozyme; Placental alkaline phosphatase 1; PLAP-1; Alkaline phosphatase, placental type; ALP |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in EDTA buffer (pH 8.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P05187 Human Alkaline phosphatase, placental type |
|---|---|
| Gene Symbol | ALPP |
| Gene Full Name | alkaline phosphatase, placental |
| Background | The protein encoded by this gene is an alkaline phosphatase, a metalloenzyme that catalyzes the hydrolysis of phosphoric acid Polyesters. It belongs to a multigene family composed of four alkaline phosphatase isoenzymes. The enzyme functions as a homodimer and has a catalytic site containing one magnesium and two zinc ions, which are required for its enzymatic function. The protein is primarily expressed in placental and endometrial tissue; however, strong ectopic expression has been detected in ovarian adenocarcinoma, serous cystadenocarcinoma, and other ovarian cancer cells. [provided by RefSeq, Jan 2015] |
| Function | Alkaline phosphatase that can hydrolyze various phosphate compounds. [UniProt] |
| Cellular Localization | Cell membrane; Lipid-anchor, GPI-anchor. [UniProt] |
| Calculated MW | 58 kDa |
| PTM | Disulfide bond; Glycoprotein; GPI-anchor; Lipoprotein |
Images (5) Click the Picture to Zoom In
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ARG43614 anti-PLAP / Placental alkaline phosphatase antibody IHC-P image
Immunohistochemistry: Human placenta ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:250 dilution.
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ARG43614 anti-PLAP / Placental alkaline phosphatase antibody ICC/IF image
Immunofluorescence: HeLa cell stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:250 dilution.
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ARG43614 anti-PLAP / Placental alkaline phosphatase antibody WB image
Western blot: Human placenta cell lysates stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:1000 dilution.
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ARG43614 anti-PLAP / Placental alkaline phosphatase antibody FACS image
Flow Cytometry: SiHa cells stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:500 / 1X10^6 cells.
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ARG43614 anti-PLAP / Placental alkaline phosphatase antibody ICC/IF image
Immunofluorescence:Human placenta stained with ARG43614 anti-PLAP / Placental alkaline phosphatase antibody at 1:250 dilution.
