ARG65251
anti-PLA2G4A antibody
anti-PLA2G4A antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody
Publication1
Overview
| Product Description | Goat Polyclonal antibody recognizes PLA2G4A |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PLA2G4A |
| Antigen Species | Human |
| Immunogen | C-QEKTFRQQRKEHIR |
| Conjugation | Un-conjugated |
| Alternate Names | Phospholipase A2 group IVA; cPLA2; cPLA2-alpha; Phosphatidylcholine 2-acylhydrolase; PLA2G4; Cytosolic phospholipase A2; EC 3.1.1.5; EC 3.1.1.4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles. [provided by RefSeq, Jul 2008] |
| Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 85 kDa |
| PTM | Activated by phosphorylation at both Ser-505 and Ser-727. |
Images (5) Click the Picture to Zoom In
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ARG65251 anti-PLA2G4A antibody WB image
Western Blot: HeLa lysate (35 µg protein in RIPA buffer) stained with ARG65251 anti-PLA2G4A antibody at 1 µg/ml dilution.
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ARG65251 anti-PLA2G4A antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed A549 cells permeabilized with 0.15% Triton. Cells were stained with ARG65251 anti-PLA2G4A antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
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ARG65251 anti-PLA2G4A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65251 anti-PLA2G4A antibody at 3.75 µg/ml dilution followed by AP-staining.
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ARG65251 anti-PLA2G4A antibody WB image
Western blot: 35 µg of A549 cell lysate (in RIPA buffer) stained with ARG65251 anti-PLA2G4A antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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ARG65251 anti-PLA2G4A antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed A549 cells permeabilized with 0.5% Triton. Cells were stained with ARG65251 anti-PLA2G4A antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
Specific References
High-Intensity Interval Training Attenuates Ketogenic Diet-Induced Liver Fibrosis in Type 2 Diabetic Mice by Ameliorating TGF-β1/Smad Signaling.
WB / Mouse
