ARG64331
anti-PLA2G1B antibody
anti-PLA2G1B antibody for Western blot and Human
Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PLA2G1B |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Dog |
| Tested Application | WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PLA2G1B |
| Antigen Species | Human |
| Immunogen | NKAHKNLDTKKYCQS |
| Conjugation | Un-conjugated |
| Alternate Names | Group IB phospholipase A2; PLA2A; Phospholipase A2; PLA2; Phosphatidylcholine 2-acylhydrolase 1B; PPLA2; EC 3.1.1.4 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Phospholipase A2 (EC 3.1.1.4) catalyzes the release of fatty acids from glycero-3-phosphocholines. The best known varieties are the digestive enzymes secreted as zymogens by the pancreas of mammals. Sequences of pancreatic PLA2 enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Other forms of PLA2 have been isolated from brain, liver, lung, spleen, intestine, macrophages, leukocytes, erythrocytes, inflammatory exudates, chondrocytes, and platelets (Seilhamer et al., 1986 [PubMed 3028739]) .[supplied by OMIM, Mar 2008] |
| Research Area | Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 16 kDa |
| PTM | Activated by trypsin cleavage in the duodenum. Can also be activated by thrombin or autocatalytically. |
Images (2) Click the Picture to Zoom In
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ARG64331 anti-PLA2G1B antibody WB image
Western Blot: Human Pancreas lysate (35 µg protein in RIPA buffer) stained with ARG64331 anti-PLA2G1B antibody at 0.01 µg/ml dilution.
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ARG64331 anti-PLA2G1B antibody WB image
Western Blot: 1). Mock transfection; 2) PLA2G1B (RC216089) expressing plasmid transfected HEK293 cell lysate standed with ARG64331 anti-PLA2G1B antibody
