ARG51804
anti-PKM2 phospho (Ser37) antibody
anti-PKM2 phospho (Ser37) antibody for ICC/IF,Western blot and Human
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Colorectal Carcinoma Marker antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PKM2 phospho (Ser37) |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PKM2 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2. |
| Conjugation | Un-conjugated |
| Alternate Names | PK3; PKM2; OIP3; Pyruvate kinase muscle isozyme; CTHBP; HEL-S-30; THBP1; OIP-3; Pyruvate kinase 2/3; Tumor M2-PK; Cytosolic thyroid hormone-binding protein; EC 2.7.1.40; Opa-interacting protein 3; p58; TCB; Pyruvate kinase PKM; Thyroid hormone-binding protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PKM |
| Gene Full Name | pyruvate kinase, muscle |
| Background | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. |
| Function | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30014 Phospho PKM2 Antibody Duo (Total, pS37) Related products: PKM2 antibodies; PKM2 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody; Colorectal Carcinoma Marker antibody |
| Calculated MW | 58 kDa |
| PTM | ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35. |
Images (2) Click the Picture to Zoom In
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ARG51804 anti-PKM2 phospho (Ser37) antibody WB image
Western blot: in vitro kinase assays carried out with puried active ERK2, wild-type (WT) PKM2 and PKM2 S37A mutant stained with ARG51804 anti-PKM2 phospho (Ser37) antibody.
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ARG51804 anti-PKM2 phospho (Ser37) antibody ICC/IF image
Immunofluorescence: methanol-fixed MEF cells stained with ARG51804 anti-PKM2 phospho (Ser37) antibody.
