ARG43957

anti-PIGS antibody

anti-PIGS antibody for ELISA,Flow cytometry,ICC/IF,Western blot and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PIGS
Tested Reactivity Hu, Rat
Tested Application ELISA, FACS, ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Target Name PIGS
Antigen Species Human
Immunogen Human PIGS recombinant protein
Expression System E.coli
Conjugation Un-conjugated
Protein Full Name GPI transamidase component PIG-S
Alternate Names PIGS; Phosphatidylinositol Glycan Anchor Biosynthesis Class S; GPI Transamidase Component PIG-S; Phosphatidylinositol-Glycan Biosynthesis Class S Protein ; Phosphatidylinositol Glycan, Class S; GPI Transamidase Subunit; PIG-S; GPIBD18; DEE95

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA0.1-0.5 µg/ml
FACS1-3 µg /1x10^6 cells
ICC/IF5 µg/ml
WB0.25-0.5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose.
Stabilizer 4% Trehalose
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Bioinformation

Database Links

GeneID: 303277 Rat PIGS

GeneID: 94005 Human PIGS

Swiss-port # Q5XI31 Rat GPI transamidase component PIG-S

Swiss-port # Q96S52 Human GPI transamidase component PIG-S

Gene Symbol PIGS
Gene Full Name Phosphatidylinositol Glycan Anchor Biosynthesis Class S
Background This gene encodes a protein that is involved in GPI-anchor biosynthesis. The glycosylphosphatidylinositol (GPI) anchor is a glycolipid found on many blood cells and serves to anchor proteins to the cell surface. This gene encodes an essential component of the multisubunit enzyme, GPI transamidase. GPI transamidase mediates GPI anchoring in the endoplasmic reticulum, by catalyzing the transfer of fully assembled GPI units to proteins.
Function Component of the GPI transamidase complex. Essential for transfer of GPI to proteins, particularly for formation of carbonyl intermediates.
Cellular Localization Endoplasmic reticulum, Membrane
Calculated MW 62 kDa
PTM Glycoprotein

Images (4) Click the Picture to Zoom In

  • ARG43957 anti-PIGS antibody WB image

    Western blot: Human hepatocellular carcinoma tumor tissue and Human hepatocellular carcinoma paracancerous stained with ARG43957 anti-PIGS antibody at 0.5 μg/mL dilution.

  • ARG43957 anti-PIGS antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG43957 anti-PIGS antibody at 5 μg/ml dilution.

  • ARG43957 anti-PIGS antibody FACS image

    Flow Cytometry: HELA cells stained with ARG43957 anti-PIGS antibody (blue) at 1 μg/1x10^6 cells dilution.

  • ARG43957 anti-PIGS antibody WB image

    Western blot: Rat liver stained with ARG43957 anti-PIGS antibody at 0.5 μg/mL dilution.