ARG57978
anti-PIEZO1 / FAM38A antibody
anti-PIEZO1 / FAM38A antibody for ICC/IF,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PIEZO1 / FAM38A |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PIEZO1 / FAM38A |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 2230-2420 of Human PIEZO1. (NP_001136336.2). |
| Conjugation | Un-conjugated |
| Alternate Names | Mib; Piezo-type mechanosensitive ion channel component 1; Membrane protein induced by beta-amyloid treatment; FAM38A; Protein FAM38A; DHS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | ~ 300 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q0KL00 Rat Piezo-type mechanosensitive ion channel component 1 Swiss-port # Q92508 Human Piezo-type mechanosensitive ion channel component 1 |
|---|---|
| Gene Symbol | PIEZO1 |
| Gene Full Name | piezo-type mechanosensitive ion channel component 1 |
| Background | The protein encoded by this gene is a mechanically-activated ion channel that links mechanical forces to biological signals. The encoded protein contains 36 transmembrane domains and functions as a homotetramer. Defects in this gene have been associated with dehydrated hereditary stomatocytosis. [provided by RefSeq, Jul 2015] |
| Function | Pore-forming subunit of a mechanosensitive non-specific cation channel. Generates currents characterized by a linear current-voltage relationship and are sensitive to ruthenium red and gadolinium. Plays a key role in epithelial cell adhesion by maintaining integrin activation through R-Ras recruitment to the ER, most probably in its activated state, and subsequent stimulation of calpain signaling. In the kidney, may contribute to the detection of intraluminal pressure changes and to urine flow sensing. Acts as shear-stress sensors that promote endothelial cell organization and alignment in the direction of blood flow. Plays a key role in blood vessel formation and vascular structure in both development and adult physiology. [UniProt] |
| Cellular Localization | Cell membrane, Cell projection, Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane, Multi-pass membrane protein, lamellipodium membrane. [UniProt] |
| Calculated MW | 287 kDa |
Images (4) Click the Picture to Zoom In
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ARG57978 anti-PIEZO1 / FAM38A antibody WB image
Western blot: HeLa cell lysate stained with ARG57978 anti-PIEZO1 / FAM38A antibody at 1:500 dilution.
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ARG57978 anti-PIEZO1 / FAM38A antibody ICC/IF image
Immunofluorescence: Human MCF7 cells stained with ARG57978 anti-PIEZO1 / FAM38A antibody at 1:100 dilution.
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ARG57978 anti-PIEZO1 / FAM38A antibody ICC/IF image
Immunofluorescence: Mouse NIH-3T3 cells stained with ARG57978 anti-PIEZO1 / FAM38A antibody at 1:100 dilution.
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ARG57978 anti-PIEZO1 / FAM38A antibody ICC/IF image
Immunofluorescence: Rat PC-12 cells stained with ARG57978 anti-PIEZO1 / FAM38A antibody at 1:100 dilution.
