ARG51810
anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Tyr199) antibody
anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Tyr199) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Immune System antibody; Signaling Transduction antibody
Publication3
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PI3 Kinase p85 / p55 phospho (Tyr467 / Tyr199) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Specificity | The antibody reacts PI3 Kinase p85 and p55 only when phosphorylated at tyrosine 467 of p85 and tyrosine 199 of p55. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PI3 Kinase p85 / p55 |
| Antigen Species | Human |
| Immunogen | Peptide sequence around phosphorylation site of Tyrosine 467 (L-Y(p)-E-E-Y) derived from Human PI3 Kinase p85 / p55. |
| Conjugation | Un-conjugated |
| Alternate Names | GRB1; PI3-kinase subunit p85-alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; IMD36; PtdIns-3-kinase regulatory subunit alpha; p85-ALPHA; p85; AGM7; PtdIns-3-kinase regulatory subunit p85-alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide. |
| Buffer | PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PIK3R1 |
| Gene Full Name | phosphoinositide-3-kinase, regulatory subunit 1 (alpha) |
| Background | Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011] |
| Function | Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling (PubMed:17626883, PubMed:19805105, PubMed:7518429). Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement (PubMed:20348923). [UniProt] |
| Highlight | Related products: PI3K P85 antibodies; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Immune System antibody; Signaling Transduction antibody |
| Calculated MW | 84 kDa |
| PTM | Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation. Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR. |
Images (1) Click the Picture to Zoom In
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ARG51810 anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Tyr199) antibody WB image
Western blot: Extracts from 293 cells untreated or treated with sorbitol stained with ARG51810 anti-PI3 Kinase p85 / p55 phospho (Tyr467 / Tyr199) antibody.
Specific References
Comprehensive analysis of clinical prognosis and biological significance of CNIH4 in cervical cancer
WB / Human
Endosulfan promotes cell migration via PTP4A3-mediated signaling pathways in HUVECs.
WB / Human
eIF4E and 4EBP1 are prognostic markers of head and neck squamous cell carcinoma recurrence after definitive surgery and adjuvant radiotherapy.
IHC-P / Human
