ARG40812

anti-PGK2 antibody

anti-PGK2 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PGK2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PGK2
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 242-360 of Human PGK2 (NP_620061.2).
Conjugation Un-conjugated
Alternate Names EC 2.7.2.3; PGKB; Phosphoglycerate kinase, testis specific; HEL-S-272; dJ417L20.2; Phosphoglycerate kinase 2; PGKPS

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat
Observed Size 44 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18663 Mouse PGK2

GeneID: 5232 Human PGK2

Swiss-port # P07205 Human Phosphoglycerate kinase 2

Swiss-port # P09041 Mouse Phosphoglycerate kinase 2

Gene Symbol PGK2
Gene Full Name phosphoglycerate kinase 2
Background This gene is intronless, arose via retrotransposition of the phosphoglycerate kinase 1 gene, and is expressed specifically in the testis. Initially assumed to be a pseudogene, the encoded protein is actually a functional phosphoglycerate kinase that catalyzes the reversible conversion of 1,3-bisphosphoglycerate to 3-phosphoglycerate, during the Embden-Meyerhof-Parnas pathway of glycolysis, in the later stages of spermatogenesis.[provided by RefSeq, May 2010]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 45 kDa

Images (2) Click the Picture to Zoom In

  • ARG40812 anti-PGK2 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human mammary cancer stained with ARG40812 anti-PGK2 antibody at 1:100 dilution.

  • ARG40812 anti-PGK2 antibody WB image

    Western blot: 25 µg of Jurkat cell lysate stained with ARG40812 anti-PGK2 antibody at 1:3000 dilution.