ARG57152
anti-PGK1 antibody [2F4]
anti-PGK1 antibody [2F4] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [2F4] recognizes PGK1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2F4 |
| Isotype | IgG1, kappa |
| Target Name | PGK1 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-417 of Human PGK1 |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.7.2.3; Primer recognition protein 2; PGKA; PRP 2; Phosphoglycerate kinase 1; MIG10; Cell migration-inducing gene 10 protein; HEL-S-68p |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PGK1 |
| Gene Full Name | phosphoglycerate kinase 1 |
| Background | The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. The encoded protein may also act as a cofactor for polymerase alpha. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Deficiency of the enzyme is associated with a wide range of clinical phenotypes hemolytic anemia and neurological impairment. Pseudogenes of this gene have been defined on chromosomes 19, 21 and the X chromosome. [provided by RefSeq, Jan 2014] |
| Function | In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein). [UniProt] |
| Calculated MW | 45 kDa |
Images (1) Click the Picture to Zoom In
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ARG57152 anti-PGK1 antibody [2F4] WB image
Western blot: 40 µg of 1) HeLa, 2) LNCaP, 3) A549, 4) HepG2, 5) 293T, 6) K562, and 7) Jurkat cell lysates stained with ARG57152 anti-PGK1 antibody [2F4] at 1:1000.
