ARG57158
anti-PGAM2 antibody [5A7]
anti-PGAM2 antibody [5A7] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [5A7] recognizes PGAM2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5A7 |
| Isotype | IgG2b, kappa |
| Target Name | PGAM2 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-253 of Human PGAM2 |
| Conjugation | Un-conjugated |
| Alternate Names | PGAM-M; Phosphoglycerate mutase isozyme M; EC 3.1.3.13; PGAMM; GSD10; BPG-dependent PGAM 2; Phosphoglycerate mutase 2; EC 5.4.2.11; EC 5.4.2.4; Muscle-specific phosphoglycerate mutase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PGAM2 |
| Gene Full Name | phosphoglycerate mutase 2 (muscle) |
| Background | Phosphoglycerate mutase (PGAM) catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway. The PGAM is a dimeric enzyme containing, in different tissues, different proportions of a slow-migrating muscle (MM) isozyme, a fast-migrating brain (BB) isozyme, and a hybrid form (MB). This gene encodes muscle-specific PGAM subunit. Mutations in this gene cause muscle phosphoglycerate mutase eficiency, also known as glycogen storage disease X. [provided by RefSeq, Sep 2009] |
| Function | Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. [UniProt] |
| Calculated MW | 29 kDa |
Images (1) Click the Picture to Zoom In
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ARG57158 anti-PGAM2 antibody [5A7] WB image
Western blot: 50 ng of 1) Recombnant protein, 40 µg of 2) HeLa, 3) HepG2, 4) 293T, 5) Jurkat, 6) NIH3T3, 7) A549, and 8) MCF7 cell lysates stained with ARG57158 anti-PGAM2 antibody [5A7] at 1:1000.
