ARG57141
anti-PGAM1 antibody [1G4]
anti-PGAM1 antibody [1G4] for Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1G4] recognizes PGAM1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1G4 |
| Isotype | IgG2a, lambda |
| Target Name | PGAM1 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-254 of Human PGAM1 |
| Conjugation | Un-conjugated |
| Alternate Names | PGAMA; EC 3.1.3.13; Phosphoglycerate mutase isozyme B; HEL-S-35; EC 5.4.2.11; PGAM-B; BPG-dependent PGAM 1; Phosphoglycerate mutase 1; EC 5.4.2.4 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PGAM1 |
| Gene Full Name | phosphoglycerate mutase 1 (brain) |
| Background | Phosphoglyceric acid mutase (EC 2.7.5.3) is widely distributed in mammalian tissues where it catalyzes the reversible reaction of 3-phosphoglycerate (3-PGA) to 2-phosphoglycerate (2-PGA) in the glycolytic pathway (summary by Chen et al., 1974 [PubMed 4811757]).[supplied by OMIM, Nov 2010] |
| Function | Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. [UniProt] |
| Calculated MW | 29 kDa |
| PTM | Acetylated at Lys-253, Lys-253 and Lys-254 under high glucose condition. Acetylation increases catalytic activity. Under glucose restriction SIRT1 levels dramatically increase and it deacetylates the enzyme. |
Images (1) Click the Picture to Zoom In
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ARG57141 anti-PGAM1 antibody [1G4] WB image
Western blot: 40 µg of 1) 293T, 2) Jurkat, 3) Raji, 4) A431, and 5) HeLa cell lysates stained with ARG57141 anti-PGAM1 antibody [1G4] at 1:1000.
