ARG56306

anti-PFKM antibody

anti-PFKM antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PFKM
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PFKM
Antigen Species Human
Immunogen Recombinant protein of Human PFKM
Conjugation Un-conjugated
Alternate Names PFK-A; 6-phosphofructokinase type A; PPP1R122; PFKX; ATP-dependent 6-phosphofructokinase, muscle type; EC 2.7.1.11; Phosphofructo-1-kinase isozyme A; PFK1; ATP-PFK; GSD7; PFK-1; Phosphohexokinase; PFK-M; PFKA; Fructose 6 Phosphate Kinase

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control 22Rv-1
Observed Size ~ 90 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18642 Mouse PFKM

GeneID: 5213 Human PFKM

GeneID: 65152 Rat PFKM

Gene Symbol PFKM
Gene Full Name phosphofructokinase, muscle
Background Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]
Function Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis. [UniProt]
Calculated MW 85 kDa
PTM GlcNAcylation decreases enzyme activity.

Images (2) Click the Picture to Zoom In

  • ARG56306 anti-PFKM antibody ICC/IF image

    Immunofluorescence: L929 cells stained with ARG56306 anti-PFKM antibody at 1:100 dilution.

  • ARG56306 anti-PFKM antibody WB image

    Western blot: 22Rv-1 cell lysate stained with ARG56306 anti-PFKM antibody.