ARG11125
anti-PEA15 antibody [4D2]
anti-PEA15 antibody [4D2] for IHC-Frozen sections,Western blot and Mouse,Rat,Cow
Overview
| Product Description | Mouse Monoclonal antibody [4D2] recognizes PEA15 |
|---|---|
| Tested Reactivity | Ms, Rat, Cow |
| Predict Reactivity | Hu |
| Tested Application | IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4D2 |
| Isotype | IgG1 |
| Target Name | PEA15 |
| Antigen Species | Human |
| Immunogen | Full-length Human PEA15. |
| Conjugation | Un-conjugated |
| Alternate Names | MAT1H; MAT1; Astrocytic phosphoprotein PEA-15; HUMMAT1H; 15 kDa phosphoprotein enriched in astrocytes; PED; PEA-15; Phosphoprotein enriched in diabetes; HMAT1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | ~ 15 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PEA15 |
| Gene Full Name | phosphoprotein enriched in astrocytes 15 |
| Background | This gene encodes a death effector domain-containing protein that functions as a negative regulator of apoptosis. The encoded protein is an endogenous substrate for protein kinase C. This protein is also overexpressed in type 2 diabetes mellitus, where it may contribute to insulin resistance in glucose uptake. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014] |
| Function | Blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. Inhibits RPS6KA3 activities by retaining it in the cytoplasm (By similarity). Inhibits both TNFRSF6- and TNFRSF1A-mediated CASP8 activity and apoptosis. Regulates glucose transport by controlling both the content of SLC2A1 glucose transporters on the plasma membrane and the insulin-dependent trafficking of SLC2A4 from the cell interior to the surface. [UniProt] |
| Cellular Localization | Cytoplasm. Note=Associated with microtubules. [UniProt] |
| Calculated MW | 15 kDa |
| PTM | Phosphorylated by protein kinase C and calcium-calmodulin-dependent protein kinase. These phosphorylation events are modulated by neurotransmitters or hormones. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG11125 anti-PEA15 antibody [4D2] IHC-Fr image
Immunohistochemistry: Frozen section of Rat brain tissue stained with ARG11125 anti-PEA15 antibody [4D2] (red) at 1:1000 dilution, and co-stained with anti-GFAP antibody (green) at 1:5000 dilution. Hoechst (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.).
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ARG11125 anti-PEA15 antibody [4D2] WB image
Western blot: Rat whole brain, Rat cerebellum, Mouse whole brain, Cow cortex and Cow cerebellum lysates stained with ARG11125 anti-PEA15 antibody [4D2] at 1:1000 dilution.
