ARG58155

anti-PDS1 / Securin antibody

Datasheet

Ordering Information

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Price (USD)

100 μl

389.00

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Datasheet

Overview

Product Description	Rabbit Polyclonal antibody recognizes PDS1 / Securin
Tested Reactivity	Hu
Tested Application	FACS, ICC/IF, IHC-P, IP, WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	PDS1 / Securin
Antigen Species	Human
Immunogen	Synthetic peptide derived from Human Securin.
Conjugation	Un-conjugated
Alternate Names	Tumor-transforming protein 1; Securin; TUTR1; Esp1-associated protein; PTTG; hPTTG; HPTTG; Pituitary tumor-transforming gene 1 protein; EAP1

Application Instructions

Application Suggestion

Tested Application	Dilution
FACS	1:50
ICC/IF	1:50 - 1:200
IHC-P	1:50 - 1:200
IP	1:30
WB	1:1000 - 1:5000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control

HeLa

Observed Size

~ 28 kDa

Properties

Form	Liquid
Purification	Affinity purified.
Buffer	PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative	0.02% Sodium azide
Stabilizer	50% Glycerol
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 9232 Human PTTG1 Swiss-port # O95997 Human Securin
Gene Symbol	PTTG1
Gene Full Name	pituitary tumor-transforming 1
Background	The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
Function	Regulatory protein, which plays a central role in chromosome stability, in the p53/TP53 pathway, and DNA repair. Probably acts by blocking the action of key proteins. During the mitosis, it blocks Separase/ESPL1 function, preventing the proteolysis of the cohesin complex and the subsequent segregation of the chromosomes. At the onset of anaphase, it is ubiquitinated, conducting to its destruction and to the liberation of ESPL1. Its function is however not limited to a blocking activity, since it is required to activate ESPL1. Negatively regulates the transcriptional activity and related apoptosis activity of TP53. The negative regulation of TP53 may explain the strong transforming capability of the protein when it is overexpressed. May also play a role in DNA repair via its interaction with Ku, possibly by connecting DNA damage-response pathways with sister chromatid separation. [UniProt]
Calculated MW	22 kDa
PTM	Phosphorylated at Ser-165 by CDK1 during mitosis. Phosphorylated in vitro by ds-DNA kinase. Ubiquitinated through 'Lys-11' linkage of ubiquitin moieties by the anaphase promoting complex (APC) at the onset of anaphase, conducting to its degradation. 'Lys-11'-linked ubiquitination is mediated by the E2 ligase UBE2C/UBCH10. [UniProt]