ARG40309

anti-PDP1 antibody

anti-PDP1 antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDP1
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDP1
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 72-260 of Human PDP1 (NP_060914.2).
Conjugation Un-conjugated
Alternate Names PDPC; PDPC 1; PDP; EC 3.1.3.43; Protein phosphatase 2C; PDH; PDP 1; PPM2C; [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial; Pyruvate dehydrogenase phosphatase catalytic subunit 1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control U-87MG, Mouse heart and Rat heart
Observed Size ~ 60 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 381511 Mouse PDP1

GeneID: 54704 Human PDP1

Swiss-port # Q3UV70 Mouse [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondria

Swiss-port # Q9P0J1 Human [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondria

Gene Symbol PDP1
Gene Full Name pyruvate dehyrogenase phosphatase catalytic subunit 1
Background Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]
Function Catalyzes the dephosphorylation and concomitant reactivation of the alpha subunit of the E1 component of the pyruvate dehydrogenase complex. [UniProt]
Cellular Localization Mitochondrion matrix. [UniProt]
Calculated MW 61 kDa

Images (2) Click the Picture to Zoom In

  • ARG40309 anti-PDP1 antibody WB image

    Western blot: 25 µg of U-87MG cell lysate stained with ARG40309 anti-PDP1 antibody at 1:3000 dilution.

  • ARG40309 anti-PDP1 antibody WB image

    Western blot: 25 µg of Rat heart lysate stained with ARG40309 anti-PDP1 antibody at 1:3000 dilution.