ARG66592

anti-PDLIM1 / CLP36 antibody

anti-PDLIM1 / CLP36 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes PDLIM1 / CLP36
Tested Reactivity Hu
Predict Reactivity Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDLIM1 / CLP36
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 30-110 of Human PDLIM1 / CLP36.
Conjugation Un-conjugated
Alternate Names Elfin; CLIM1; C-terminal LIM domain protein 1; hCLIM1; PDZ and LIM domain protein 1; CLP36; CLP-36; LIM domain protein CLP-36; HEL-S-112

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
IHC-P1:100 - 1:300
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 36 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9124 Human PDLIM1

Swiss-port # O00151 Human PDZ and LIM domain protein 1

Gene Symbol PDLIM1
Gene Full Name PDZ and LIM domain 1
Background This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012]
Function Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. [UniProt]
Cellular Localization Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line. Note=Associates with actin stress fibers. [UniProt]
Calculated MW 36 kDa

Images (3) Click the Picture to Zoom In

  • ARG66592 anti-PDLIM1 / CLP36 antibody ICC/IF image

    Immunofluorescence: HepG2 cells stained with ARG66592 anti-PDLIM1 / CLP36 antibody. The picture on the right is blocked with the synthetic peptide.

  • ARG66592 anti-PDLIM1 / CLP36 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human heart tissue stained with ARG66592 anti-PDLIM1 / CLP36 antibody. The picture on the right is blocked with the synthetic peptide.

  • ARG66592 anti-PDLIM1 / CLP36 antibody WB image

    Western blot: K562 cell lysate stained with ARG66592 anti-PDLIM1 / CLP36 antibody at 1:1000 dilution.