ARG63578
anti-PDLIM1 / CLP36 antibody
anti-PDLIM1 / CLP36 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Gene Regulation antibody; Metabolism antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PDLIM1 / CLP36 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDLIM1 / CLP36 |
| Antigen Species | Human |
| Immunogen | C-TPPEGYEVVTVFPK |
| Conjugation | Un-conjugated |
| Alternate Names | Elfin; CLIM1; C-terminal LIM domain protein 1; hCLIM1; PDZ and LIM domain protein 1; CLP36; CLP-36; LIM domain protein CLP-36; HEL-S-112 |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PDLIM1 |
| Gene Full Name | PDZ and LIM domain 1 |
| Background | This gene encodes a member of the enigma protein family. The protein contains two protein interacting domains, a PDZ domain at the amino terminal end and one to three LIM domains at the carboxyl terminal. It is a cytoplasmic protein associated with the cytoskeleton. The protein may function as an adapter to bring other LIM-interacting proteins to the cytoskeleton. Pseudogenes associated with this gene are located on chromosomes 3, 14 and 17. [provided by RefSeq, Oct 2012] |
| Function | Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton (PubMed:10861853). Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity). [UniProt] |
| Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody |
| Calculated MW | 36 kDa |
Images (2) Click the Picture to Zoom In
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ARG63578 anti-PDLIM1 / CLP36 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63578 anti-PDLIM1 / CLP36 antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63578 anti-PDLIM1 / CLP36 antibody WB image
Western blot: 35 µg of Human lung lysate (in RIPA buffer) stained with ARG63578 anti-PDLIM1 / CLP36 antibody at 0.1 µg/ml dilution and incubated at RT for 1 hour.
