ARG55699
anti-PDK2 antibody
anti-PDK2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PDK2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDK2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human PDK2. |
| Conjugation | Un-conjugated |
| Alternate Names | PDHK2; PDKII; [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial; EC 2.7.11.2; Pyruvate dehydrogenase kinase isoform 2; PDH kinase 2; PDKII |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Rat heart |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide. |
| Preservative | 0.09% (W/V) Sodium azide. |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PDK2 |
| Gene Full Name | pyruvate dehydrogenase kinase, isozyme 2 |
| Background | This gene encodes a member of the pyruvate dehydrogenase kinase family. The encoded protein phosphorylates pyruvate dehydrogenase, down-regulating the activity of the mitochondrial pyruvate dehydrogenase complex. Overexpression of this gene may play a role in both cancer and diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2010] |
| Function | Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis. [UniProt] |
| Cellular Localization | Mitochondrion matrix. |
| Calculated MW | 46 kDa |
Images (3) Click the Picture to Zoom In
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ARG55699 anti-PDK2 antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded skeletal muscle stained with ARG55699 anti-PDK2 antibody.
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ARG55699 anti-PDK2 antibody WB image
Western blot: 35 µg of Rat heart lysate stained with ARG55699 anti-PDK2 antibody.
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ARG55699 anti-PDK2 antibody FACS image
Flow Cytometry: Jurkat cells stained with ARG55699 anti-PDK2 antibody (bottom histogram) or without primary antibody as control (top histogram), followed by incubation with FITC labelled secondary antibody.
