ARG22213
anti-PDIA3 / ERp57 antibody [MaP.Erp57]
anti-PDIA3 / ERp57 antibody [MaP.Erp57] for ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine,Dog,Guinea pig,Hamster,Monkey,Pig,Rabbit
Overview
| Product Description | Mouse Monoclonal antibody [Map.ERP57] recognizes PDIA3 / ERp57 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Dog, Gpig, Hm, Mk, Pig, Rb |
| Tested Application | ICC/IF, IHC, IP, WB |
| Specificity | Detects ~57kDa. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Map.ERP57 |
| Isotype | IgG1 |
| Target Name | PDIA3 / ERp57 |
| Antigen Species | Human |
| Immunogen | Human recombinant ERp57 |
| Conjugation | Un-conjugated |
| Alternate Names | EC 5.3.4.1; Disulfide isomerase ER-60; HEL-S-93n; GRP57; p58; Endoplasmic reticulum resident protein 60; ER protein 57; ER protein 60; ERp57; GRP58; P58; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; ER60; HEL-S-269; Protein disulfide-isomerase A3; PI-PLC; ERp60; ERp61; HsT17083; Endoplasmic reticulum resident protein 57 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PDIA3 |
| Gene Full Name | protein disulfide isomerase family A, member 3 |
| Background | This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008] |
| Cellular Localization | Endoplasmic Reticulum, Endoplasmic reticulum lumen, Melanosome |
| Highlight | Related products: ERp57 antibodies; Anti-Mouse IgG secondary antibodies; Related poster download: Organelle Markers & Loading Control |
| Calculated MW | 57 kDa |
Images (2) Click the Picture to Zoom In
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ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
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ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] WB image
Western blot: Human cell lysates stained with ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] at 1:1000 dilution.
