ARG55326

anti-PDIA3 / ERp57 antibody

anti-PDIA3 / ERp57 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes PDIA3 / ERp57
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDIA3 / ERp57
Antigen Species Human
Immunogen Recombinant protein of Human PDIA3 (NP_005304.3)
Conjugation Un-conjugated
Alternate Names EC 5.3.4.1; Disulfide isomerase ER-60; HEL-S-93n; GRP57; p58; Endoplasmic reticulum resident protein 60; ER protein 57; ER protein 60; ERp57; GRP58; P58; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; ER60; HEL-S-269; Protein disulfide-isomerase A3; PI-PLC; ERp60; ERp61; HsT17083; Endoplasmic reticulum resident protein 57

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Jurkat

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14827 Mouse PDIA3

GeneID: 2923 Human PDIA3

GeneID: 29468 Rat PDIA3

Gene Symbol PDIA3
Gene Full Name protein disulfide isomerase family A, member 3
Background This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 57 kDa

Images (3) Click the Picture to Zoom In

  • ARG55326 anti-PDIA3 / ERp57 antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG55326 anti-PDIA3 / ERp57 antibody.

  • ARG55326 anti-PDIA3 / ERp57 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Mouse brain tissue stained with ARG55326 anti-PDIA3 / ERp57 antibody at 1:100 dilution.

  • ARG55326 anti-PDIA3 / ERp57 antibody WB image

    Western blot: Jurkat cell lysate stained with ARG55326 anti-PDIA3 / ERp57 antibody.