ARG10805
anti-PDE5A antibody
anti-PDE5A antibody for Confocal microscopy (Confocal),Dot blot,ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Pig
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PDE5A |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Pig |
| Tested Application | Confocal, Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDE5A |
| Antigen Species | Human |
| Immunogen | Synthetic peptide taken within aa. 120-170 from Human PDE5A. |
| Conjugation | Un-conjugated |
| Alternate Names | cGMP-specific 3',5'-cyclic phosphodiesterase; CN5A; CGB-PDE; cGMP-binding cGMP-specific phosphodiesterase; PDE5; EC 3.1.4.35 |
Application Instructions
| Application Suggestion |
|
||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 30% Glycerol and 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PDE5A |
| Gene Full Name | phosphodiesterase 5A, cGMP-specific |
| Background | This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| Function | Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP. [UniProt] |
| Calculated MW | 100 kDa |
| PTM | Phosphorylation is regulated by binding of cGMP to the two allosteric sites (By similarity). Phosphorylation by PRKG1 leads to its activation. |
Images (2) Click the Picture to Zoom In
-
ARG10805 anti-PDE5A antibody WB image
Western blot: Human PDE5A-GFP overexpressing cells untreated or treated with ANP. The blots were stained with ARG10805 anti-PDE5A antibody at 1:500 dilution.
-
ARG10805 anti-PDE5A antibody Confocal image
Confocal microscopy: Cells expressing PDE5A stained with ARG10805 anti-PDE5A antibody at 1:200 dilution.
