ARG10819
anti-PDE4D phospho (Ser190) antibody
anti-PDE4D phospho (Ser190) antibody for Dot blot,ELISA,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PDE4D phospho (Ser190) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | Dot, ELISA, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDE4D |
| Antigen Species | Human |
| Immunogen | Synthetic peptide selective for PDE4D PKA site taken within aa. 50-100 from Human PDE4D. Derived around the PKA phosphorylation site of Ser190: R-E-Sp-F-L. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.1.4.53; STRK1; DPDE3; PDE43; ACRDYS2; PDE4DN2; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; HSPDE4D |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 30% Glycerol and 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PDE4D |
| Gene Full Name | phosphodiesterase 4D, cAMP-specific |
| Background | This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009] |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. [UniProt] |
| Calculated MW | 91 kDa |
| PTM | Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation. |
Images (1) Click the Picture to Zoom In
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ARG10819 anti-PDE4D phospho (Ser190) antibody WB image
Western blot: Non-phosphorylated and phosphorylated PDE4D protein stained with ARG10819 anti-PDE4D phospho (Ser190) antibody and ARG10803 anti-PDE4D antibody at 1:500 dilution.
