ARG63896
anti-PDE4B antibody
anti-PDE4B antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PDE4B |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognise all reported isoforms (NP_002591.2, NP_001032418.1, NP_001032416.1 and NP_001032417.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDE4B |
| Antigen Species | Human |
| Immunogen | C-DIDIATEDKSPVDT |
| Conjugation | Un-conjugated |
| Alternate Names | cAMP-specific 3',5'-cyclic phosphodiesterase 4B; EC 3.1.4.53; PDEIVB; DPDE4; PDE32 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q07343 Human cAMP-specific 3',5'-cyclic phosphodiesterase 4B |
|---|---|
| Background | This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Cyclic nucleotides are important second messengers that regulate and mediate a number of cellular responses to extracellular signals, such as hormones, light, and neurotransmitters. The cyclic nucleotide phosphodiesterases (PDEs) regulate the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. This gene encodes a protein that specifically hydrolyzes cAMP. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 83 kDa |
Images (3) Click the Picture to Zoom In
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ARG63896 anti-PDE4B antibody WB image
Western blot: Mouse Brain Lysate (35 µg protein in RIPA buffer) stained with ARG63896 anti-PDE4B antibody at 0.5 µg/ml dilution.
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ARG63896 anti-PDE4B antibody WB image
Western blot: 1). Mock transfection; 2) Human PDE4B (RC211956) expressing plasmid transfected HEK293 cell lysate standed with ARG63896 anti-PDE4B antibody.
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ARG63896 anti-PDE4B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63896 anti-PDE4B antibody at 5 µg/ml dilution followed by AP-staining.
