ARG10801
anti-PDE4B antibody
anti-PDE4B antibody for Confocal microscopy (Confocal),Dot blot,ELISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PDE4B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | Confocal, Dot, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDE4B |
| Antigen Species | Human |
| Immunogen | Synthetic peptide common to all PDE4B subtypes taken near the N-terminus region. |
| Conjugation | Un-conjugated |
| Alternate Names | cAMP-specific 3',5'-cyclic phosphodiesterase 4B; EC 3.1.4.53; PDEIVB; DPDE4; PDE32 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 30% Glycerol and 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P14646 Rat cAMP-specific 3',5'-cyclic phosphodiesterase 4B Swiss-port # Q07343 Human cAMP-specific 3',5'-cyclic phosphodiesterase 4B |
|---|---|
| Gene Symbol | PDE4B |
| Gene Full Name | phosphodiesterase 4B, cAMP-specific |
| Background | This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014] |
| Function | Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents. [UniProt] |
| Calculated MW | 83 kDa |
Images (4) Click the Picture to Zoom In
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ARG10801 anti-PDE4B antibody IHC-Fr image
Immunohistochemistry: Frozen section of seminiferous tubule stained with ARG10801 anti-PDE4B antibody.
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ARG10801 anti-PDE4B antibody WB image
Western blot: 1) Recombinant PDE4B variants, 2) Recombinant PDE4B3, and 3) Rat testis stained with ARG10801 anti-PDE4B antibody at 1:500 dilution.
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ARG10801 anti-PDE4B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian medulla tissue stained with ARG10801 anti-PDE4B antibody at 1:300 dilution.
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ARG10801 anti-PDE4B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human neuroblastoma (SHSY5Y) tissue stained with ARG10801 anti-PDE4B antibody at 1:250 dilution.
