ARG65163
anti-PDE1A antibody
anti-PDE1A antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PDE1A |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize isoform 1 (NP_005010.2) only. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PDE1A |
| Antigen Species | Human |
| Immunogen | C-DLHKNSEDLVNAE |
| Conjugation | Un-conjugated |
| Alternate Names | CAM-PDE-1A; Cam-PDE 1A; HCAM1; Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A; EC 3.1.4.17; HSPDE1A; hCam-1; HCAM-1; 61 kDa Cam-PDE |
Application Instructions
| Application Suggestion |
|
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P54750 Human Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1A |
|---|---|
| Background | Cyclic nucleotide phosphodiesterases (PDEs) play a role in signal transduction by regulating intracellular cyclic nucleotide concentrations through hydrolysis of cAMP and/or cGMP to their respective nucleoside 5-prime monophosphates. Members of the PDE1 family, such as PDE1A, are Ca(2+)/calmodulin (see CALM1; MIM 114180)-dependent PDEs (CaM-PDEs) that are activated by calmodulin in the presence of Ca(2+) (Michibata et al., 2001 [PubMed 11342109]; Fidock et al., 2002 [PubMed 11747989]).[supplied by OMIM, Oct 2009] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 61 kDa |
Images (2) Click the Picture to Zoom In
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ARG65163 anti-PDE1A antibody WB image
Western Blot: Human Cerebellum lysate (35 µg protein in RIPA buffer) stained with ARG65163 anti-PDE1A antibody at 1 µg/ml dilution.
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ARG65163 anti-PDE1A antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65163 anti-PDE1A antibody at 3.75 µg/ml dilution followed by AP-staining.
