ARG44459
anti-PCMT1 antibody
anti-PCMT1 antibody for IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Western blot,Flow cytometry and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PCMT1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | PCMT1 |
| Antigen Species | Human |
| Immunogen | Human PCMT1 recombinant protein |
| Conjugation | Un-conjugated |
| Alternate Names | PCMT1; Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase; Protein-L-Isoaspartate(D-Aspartate) O-Methyltransferase; Protein L-Isoaspartyl/D-Aspartyl Methyltransferase; L-Isoaspartyl Protein Carboxyl Methyltransferase; Protein-Beta-Aspartate Methyltransferase; EC 2.1.1.77; PIMT; Epididymis Secretory Sperm Binding Protein |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links |
Swiss-port # P22061 Human Protein-L-isoaspartate(D-aspartate) O-methyltransferase Swiss-port # P22062 Rat Protein-L-isoaspartate(D-aspartate) O-methyltransferase |
|---|---|
| Gene Symbol | PCMT1 |
| Gene Full Name | Protein-L-Isoaspartate (D-Aspartate) O-Methyltransferase |
| Background | This gene encodes a member of the type II class of protein carboxyl methyltransferase enzymes. The encoded enzyme plays a role in protein repair by recognizing and converting D-aspartyl and L-isoaspartyl residues resulting from spontaneous deamidation back to the normal L-aspartyl form. The encoded protein may play a protective role in the pathogenesis of Alzheimer's disease, and single nucleotide polymorphisms in this gene have been associated with spina bifida and premature ovarian failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. |
| Function | Initiates the repair of damaged proteins by catalyzing methyl esterification of L-isoaspartyl and D-aspartyl residues produced by spontaneous isomerization and racemization of L-aspartyl and L-asparaginyl residues in aging peptides and proteins . |
| Cellular Localization | Cytoplasm |
| Calculated MW | 25 kDa |
| PTM | Acetylation |
Images (5) Click the Picture to Zoom In
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ARG44459 anti-PCMT1 antibody IHC-P image
Immunohistochemistry: Human glioblastoma stained with ARG44459 anti-PCMT1 antibody at 2 μg/mL dilution.
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ARG44459 anti-PCMT1 antibody ICC/IF image
Immunofluorescence: SiHa stained with ARG44459 anti-PCMT1 antibody at 5 μg/mL dilution.
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ARG44459 anti-PCMT1 antibody WB image
Western blot: Hela, Raji and 293T stained with ARG44459 anti-PCMT1 antibody at 0.5 μg/mL dilution.
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ARG44459 anti-PCMT1 antibody WB image
Western blot: Rat testis and Rat brain stained with ARG44459 anti-PCMT1 antibody at 0.5 μg/mL dilution.
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ARG44459 anti-PCMT1 antibody WB image
Western blot: Mouse testis and Mouse brain stained with ARG44459 anti-PCMT1 antibody at 0.5 μg/mL dilution.
