ARG63857
anti-PCK2 / PEPCKM antibody
anti-PCK2 / PEPCKM antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PCK2 / PEPCKM |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog, Pig |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PCK2 / PEPCKM |
| Antigen Species | Human |
| Immunogen | KPWKPGDKEPCAH |
| Conjugation | Un-conjugated |
| Alternate Names | PEPCK-M; Phosphoenolpyruvate carboxykinase [GTP], mitochondrial; PEPCK; EC 4.1.1.32; PEPCK2 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q16822 Human Phosphoenolpyruvate carboxykinase [GTP], mitochondrial |
|---|---|
| Background | This gene encodes a member of the phosphoenolpyruvate carboxykinase (GTP) family. The protein is a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of GTP. A cytosolic form encoded by a different gene has also been characterized and is the key enzyme of gluconeogenesis in the liver. The encoded protein may serve a similar function, although it is constitutively expressed and not modulated by hormones such as glucagon and insulin that regulate the cytosolic form. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Metabolism antibody; Signaling Transduction antibody |
| Calculated MW | 71 kDa |
Images (1) Click the Picture to Zoom In
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ARG63857 anti-PCK2 / PEPCKM antibody WB image
Western blot: Human Liver lysate (35 µg protein in RIPA buffer) stained with ARG63857 anti-PCK2 / PEPCKM antibody at 2 µg/ml dilution.
