ARG56518

anti-PCK1 / PEPCKC antibody

anti-PCK1 / PEPCKC antibody for Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PCK1 / PEPCKC
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PCK1 / PEPCKC
Antigen Species Mouse
Immunogen Synthetic peptide around the N-terminus of Mouse PCK1 / PEPCK-C.
Conjugation Un-conjugated
Alternate Names Phosphoenolpyruvate carboxykinase, cytosolic [GTP]; EC 4.1.1.32; PEPCK1; PEPCK-C; PEPCKC

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:200
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer TBS (pH 7.4), 0.02% Sodium azide, 50% Glycerol and 0.1% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18534 Mouse PCK1

GeneID: 362282 Rat PCK1

Swiss-port # P07379 Rat Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Swiss-port # Q9Z2V4 Mouse Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene Symbol Pck1
Gene Full Name phosphoenolpyruvate carboxykinase 1, cytosolic
Background This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized. [provided by RefSeq, Jul 2008]
Function Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle. [UniProt]
Calculated MW 69 kDa
PTM Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2.
Ubiquitination by UBR5 leads to proteasomal degradation.