Overview

Product Description	Rabbit Polyclonal antibody recognizes PCGF6
Tested Reactivity	Hu, Rat
Predict Reactivity	Ms
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	PCGF6
Antigen Species	Human
Immunogen	KLH-conjugated synthetic peptide corresponding to aa. 190-217 of Human PCGF6.
Conjugation	Un-conjugated
Alternate Names	RING finger protein 134; Mel18 and Bmi1-like RING finger; RNF134; Polycomb group RING finger protein 6; MBLR

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	Rat testis

Properties

Form	Liquid
Purification	Purification with Protein A and immunogen peptide.
Buffer	PBS and 0.09% (W/V) Sodium azide.
Preservative	0.09% (W/V) Sodium azide.
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 309457 Rat PCGF6 GeneID: 84108 Human PCGF6 Swiss-port # Q5XI70 Rat Polycomb group RING finger protein 6 Swiss-port # Q9BYE7 Human Polycomb group RING finger protein 6
Gene Symbol	PCGF6
Gene Full Name	polycomb group ring finger 6
Background	The protein encoded by this gene contains a RING finger motif, which is most closely related to those of polycomb group (PcG) proteins RNF110/MEL-18 and BMI1. PcG proteins are known to form protein complexes and function as transcription repressors. This protein has been shown to interact with some PcG proteins and act as a transcription repressor. The activity of this protein is found to be regulated by cell cycle dependent phosphorylation. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
Function	Transcriptional repressor. May modulate the levels of histone H3K4Me3 by activating KDM5D histone demethylase. Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. [UniProt]
Cellular Localization	Nucleus. [UniProt]
Calculated MW	39 kDa
PTM	Phosphorylated during mitosis. Phosphorylated on Ser-30 by CDK7 in vitro. [UniProt]

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