Overview

Product Description	Rabbit Polyclonal antibody recognizes PCBP1
Tested Reactivity	Hu
Predict Reactivity	Ms, Bov, Rb
Tested Application	WB
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Target Name	PCBP1
Antigen Species	Human
Immunogen	KLH-conjugated synthetic peptide corresponding to aa. 188-217 (Center) of Human PCBP1.
Conjugation	Un-conjugated
Alternate Names	HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1; Poly(rC)-binding protein 1; Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; hnRNP E1; Nucleic acid-binding protein SUB2.3

Application Instructions

Application Suggestion	Tested Application Dilution WB 1:1000
Application Note	* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control	K562

Properties

Form	Liquid
Purification	Purification with Protein A and immunogen peptide.
Buffer	PBS and 0.09% (W/V) Sodium azide.
Preservative	0.09% (W/V) Sodium azide.
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 5093 Human PCBP1 Swiss-port # Q15365 Human Poly(rC)-binding protein 1
Gene Symbol	PCBP1
Gene Full Name	poly(rC) binding protein 1
Background	This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008]
Function	Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. [UniProt]
Cellular Localization	Nucleus. Cytoplasm. Note=Loosely bound in the nucleus. May shuttle between the nucleus and the cytoplasm
Calculated MW	37 kDa
PTM	Phosphorylated; lowers poly(rC)-binding activity.

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