ARG45409
anti-PCBP1 antibody
anti-PCBP1 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PCBP1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PCBP1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human PCBP1. |
| Conjugation | Un-conjugated |
| Alternate Names | HNRPX; HNRPE1; hnRNP-X; HEL-S-85; hnRNP-E1; Poly(rC)-binding protein 1; Alpha-CP1; Heterogeneous nuclear ribonucleoprotein E1; hnRNP E1; Nucleic acid-binding protein SUB2.3 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 40 kDa |
Properties
| Form | Powder |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.005% Sodium azide and 4% Trehalose. |
| Preservative | 0.005% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PCBP1 |
| Gene Full Name | poly(rC) binding protein 1 |
| Background | This intronless gene is thought to have been generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 have paralogues (PCBP3 and PCBP4) which are thought to have arisen as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly(rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. [provided by RefSeq, Jul 2008] |
| Function | Single-stranded nucleic acid binding protein that binds preferentially to oligo dC. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. [UniProt] |
| Calculated MW | 37 kDa |
| PTM | Phosphorylated; lowers poly(rC)-binding activity. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45409 anti-PCBP1 antibody IHC-P image
Immunohistochemistry: Human breast cancer stained with ARG45409 anti-PCBP1 antibody at 2 μg/ml dilution.
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ARG45409 anti-PCBP1 antibody WB image
Western blot: HepG2 stained with ARG45409 anti-PCBP1 antibody at 0.5 μg/ml dilution.
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ARG45409 anti-PCBP1 antibody FACS image
Flow Cytometry: CACO-2 stained with ARG45409 anti-PCBP1 antibody at 1 µg/10^6 cells dilution.
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ARG45409 anti-PCBP1 antibody WB image
Western blot: NIH/3T3 stained with ARG45409 anti-PCBP1 antibody at 0.5 μg/ml dilution.
