ARG66821
anti-PAX8 antibody [SQab20233]
anti-PAX8 antibody [SQab20233] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab20233] recognizes PAX8 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab20233 |
| Isotype | IgG |
| Target Name | PAX8 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human PAX8. |
| Conjugation | Un-conjugated |
| Alternate Names | Paired box protein Pax-8 |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT (18°C - 25°C) for 30 minutes. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PAX8 |
| Gene Full Name | paired box 8 |
| Background | This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Mar 2010] |
| Function | Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 48 kDa |
Images (3) Click the Picture to Zoom In
-
ARG66821 anti-PAX8 antibody [SQab20233] WB image (Customer's Feedback)
Western blot: MCF7 cell lysate stained with ARG66821 anti-PAX8 antibody [SQab20233] at 1:1000 dilution.
-
ARG66821 anti-PAX8 antibody [SQab20233] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human thyroid cancer tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66821 anti-PAX8 antibody [SQab20233].
-
ARG66821 anti-PAX8 antibody [SQab20233] IHC-P image
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded Human serous cystadenocarcinoma of the ovary tissue. Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). The tissue section was stained with ARG66821 anti-PAX8 antibody [SQab20233].
Customer's Feedback
Excellent
Review for anti-PAX8 antibody [SQab20233]
Application：WB
Sample：MCF7
Sample Loading Amount：20 μg
SDS Page Type：Denatured
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：HRP
