ARG64256
anti-PAX6 antibody
anti-PAX6 antibody for Western blot and Mouse
Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PAX6 |
|---|---|
| Tested Reactivity | Ms |
| Predict Reactivity | Hu, Rat |
| Tested Application | WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_000271.1 and NP_001595.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PAX6 |
| Antigen Species | Human |
| Immunogen | C-REEKLRNQRRQASN |
| Conjugation | Un-conjugated |
| Alternate Names | MGDA; Aniridia type II protein; AN; Oculorhombin; Paired box protein Pax-6; AN2; WAGR; FVH1; D11S812E |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012] |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody |
| Calculated MW | 47 kDa |
| PTM | Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation. |
Images (1) Click the Picture to Zoom In
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ARG64256 anti-PAX6 antibody WB image
Western Blot: Mouse Eye lysate (35 µg protein in RIPA buffer) stained with ARG64256 anti-PAX6 antibody at 0.5 µg/ml dilution.
