ARG59986
anti-PASK antibody
anti-PASK antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
Product Description | Rabbit Polyclonal antibody recognizes PASK |
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Tested Reactivity | Hu, Ms, Rat |
Tested Application | ICC/IF, IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | PASK |
Antigen Species | Human |
Immunogen | Recombinant fusion protein corresponding to aa. 1194-1323 of Human PASK (NP_055963.2). |
Conjugation | Un-conjugated |
Alternate Names | PAS domain-containing serine/threonine-protein kinase; hPASK; PASKIN; STK37; PAS-kinase; EC 2.7.11.1 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
Positive Control | Jurkat | ||||||||
Observed Size | 190 kDa |
Properties
Form | Liquid |
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Purification | Affinity purified. |
Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 50% Glycerol |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q8CEE6 Mouse PAS domain-containing serine/threonine-protein kinase Swiss-port # Q96RG2 Human PAS domain-containing serine/threonine-protein kinase |
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Gene Symbol | PASK |
Gene Full Name | PAS domain containing serine/threonine kinase |
Background | This gene encodes a member of the serine/threonine kinase family that contains two PAS domains. Expression of this gene is regulated by glucose, and the encoded protein plays a role in the regulation of insulin gene expression. Downregulation of this gene may play a role in type 2 diabetes. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2011] |
Function | Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis: regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate to respiratory regulation. [UniProt] |
Cellular Localization | Cytoplasm. Nucleus. Note=Localizes in the nucleus of testis germ cells and in the midpiece of sperm tails. [UniProt] |
Calculated MW | 143 kDa |
PTM | Autophosphorylated on Thr-1161 and Thr-1165. Autophosphorylation is activated by phospholipids. [UniProt] |
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