ARG45499
anti-PARP2 antibody
anti-PARP2 antibody for Flow cytometry,ICC/IF,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PARP2 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | FACS, ICC/IF, WB |
| Specificity | PARP2 |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PARP2 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human PARP2. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.4.2.30; hPARP-2; ARTD2; NAD; pADPRT-2; PARP-2; Poly [ADP-ribose] polymerase 2; Poly[ADP-ribose] synthase 2; ADP-ribosyltransferase diphtheria toxin-like 2; ADPRT-2; ADPRTL2; ADPRTL3; ADPRT2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 66 kDa |
Properties
| Form | Powder |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.01% Sodium azide and 4% Trehalose. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PARP2 |
| Gene Full Name | poly (ADP-ribose) polymerase 2 |
| Background | This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008] |
| Function | Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231, PubMed:28190768, PubMed:25043379). Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed:25043379). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:10364231). Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed:28190768). In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (PubMed:27471034). [UniProt] |
| Cellular Localization | Chromosome; Nucleus. [UniProt] |
| Calculated MW | 66 kDa |
| PTM | Acetylation; ADP-ribosylation; Phosphoprotein. [UniProt] |
Images (4) Click the Picture to Zoom In
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ARG45499 anti-PARP2 antibody ICC/IF image
Immunofluorescence: HepG2 stained with ARG45499 anti-PARP2 antibody at 5 μg/ml dilution.
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ARG45499 anti-PARP2 antibody WB image
Western blot: HEK293 stained with ARG45499 anti-PARP2 antibody at 0.5 μg/ml dilution.
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ARG45499 anti-PARP2 antibody FACS image
Flow Cytometry: HL-60 stained with ARG45499 anti-PARP2 antibody at 1 µg/10^6 cells dilution.
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ARG45499 anti-PARP2 antibody WB image
Western blot: Rat testis stained with ARG45499 anti-PARP2 antibody at 0.5 μg/ml dilution.
