ARG57124
anti-PARP2 antibody [29G4]
anti-PARP2 antibody [29G4] for Flow cytometry,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [29G4] recognizes PARP2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 29G4 |
| Isotype | IgG2a, kappa |
| Target Name | PARP2 |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 233-583 of Human PARP2 |
| Conjugation | Un-conjugated |
| Alternate Names | EC 2.4.2.30; hPARP-2; ARTD2; NAD; pADPRT-2; PARP-2; Poly [ADP-ribose] polymerase 2; Poly[ADP-ribose] synthase 2; ADP-ribosyltransferase diphtheria toxin-like 2; ADPRT-2; ADPRTL2; ADPRTL3; ADPRT2 |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PARP2 |
| Gene Full Name | poly (ADP-ribose) polymerase 2 |
| Background | This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008] |
| Function | Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. [UniProt] |
| Calculated MW | 66 kDa |
| PTM | Poly-ADP-ribosylated by PARP1. Acetylation reduces DNA binding and enzymatic activity. |
Images (2) Click the Picture to Zoom In
-
ARG57124 anti-PARP2 antibody [29G4] FACS image
Flow Cytometry: U87MG cell line stained with ARG57124 anti-PARP2 antibody [29G4] at 2-5 µg for 1x10^6 cells (red line). Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate. Isotype control antibody: Mouse IgG (black line).
-
ARG57124 anti-PARP2 antibody [29G4] WB image
Western blot: 40 µg of 1) Raji, and 2) NIH-3T3 cell lysates stained with ARG57124 anti-PARP2 antibody [29G4] at 1:500.
