ARG63279
anti-PARK7 / DJ1 antibody
anti-PARK7 / DJ1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PARK7 / DJ1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Variants (NP_001116849.1; NP_009193.2) encode the same protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PARK7 / DJ1 |
| Antigen Species | Human |
| Immunogen | C-AAQVKAPLVLKD |
| Conjugation | Un-conjugated |
| Alternate Names | DJ1; DJ-1; Oncogene DJ1; EC 3.5.1.-; Parkinson disease protein 7; HEL-S-67p; EC 3.1.2.-; Protein deglycase DJ-1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: PARK7 antibodies; Anti-Goat IgG secondary antibodies; Related news: Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| Research Area | Cancer antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 20 kDa |
| PTM | Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity. Cys-106 is easily oxidized to sulfinic acid. Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress. |
Images (5) Click the Picture to Zoom In
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ARG63279 anti-PARK7 / DJ1 antibody IHC-P image
Immunohistochemistry: Human Testis stained with ARG63279 anti-PARK7 / DJ1 antibody at 7 µg/ml dilution.
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ARG63279 anti-PARK7 / DJ1 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG63279 anti-PARK7 / DJ1 antibody (green) at 5 µg/ml dilution. Nuclei Counterstained with DAPI (blue).
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ARG63279 anti-PARK7 / DJ1 antibody WB image
Western blot: 35 µg of HeLa and Jurkat cell lysates (in RIPA buffer) stained with ARG63279 anti-PARK7 / DJ1 antibody at 0.001 µg/ml dilution and incubated at RT for 1 hour.
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ARG63279 anti-PARK7 / DJ1 antibody ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG63279 anti-PARK7 / DJ1 antibody (green) at 5 µg/ml dilution. Nuclei Counterstained with DAPI (blue).
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ARG63279 anti-PARK7 / DJ1 antibody WB image
Western blot: 35 µg of Human Cerebellum, Human Frontal Cortex, Human Hippocampus, Mouse Fetal Brain, Mouse Brain and Rat Brain lysates (in RIPA buffer) stained with ARG63279 anti-PARK7 / DJ1 antibody at 0.001 µg/ml dilution and incubated at RT for 1 hour.
