ARG43886
anti-PAN2 antibody
anti-PAN2 antibody for ELISA,Flow cytometry,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PAN2 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PAN2 |
| Antigen Species | Human |
| Immunogen | Human PAN2 recombinant protein |
| Expression System | E.coli |
| Conjugation | Un-conjugated |
| Protein Full Name | PAN2-PAN3 deadenylation complex catalytic subunit PAN2 |
| Alternate Names | PAN2; Poly(A) Specific Ribonuclease Subunit PAN2; KIAA0710; USP52; PAN2-PAN3 Deadenylation Complex Catalytic Subunit PAN2; Inactive Ubiquitin Carboxyl-Terminal Hydrolase 52; Poly(A)-Nuclease Deadenylation Complex Subunit 2; PAB1P-Dependent Poly(A)-Specific Ribonuclease; PAN Deadenylation Complex Subunit 2; Ubiquitin Specific Peptidase 52; Ubiquitin Specific Protease 52; EC 3.1.13.4; HPAN2; PAN2 Homolog, PABP1 Dependent Poly A Specific Ribonuclease Subunit (S. Cerevisiae); PAN2 Poly(A) Specific Ribonuclease Subunit Homolog (S. Cerevisiae); PAB-Dependent Poly(A)-Specific Ribonuclease Subunit PAN2; PAB-Dependent Poly(A)-Specific Ribonuclease Subunit 2; PAN2 Poly(A) Specific Ribonuclease Subunit Homolog; PAN Deadenylation Complex Catalytic Subunit 2; PAN2 Poly(A) Specific Ribonuclease Subunit; PABP-Dependent Poly(A) Nuclease 2; PAB1P-Dependent Poly(A)-Nuclease |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | 0.9% NaCl, 0.2% Na2HPO4 and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PAN2 |
| Gene Full Name | Poly(A) Specific Ribonuclease Subunit PAN2 |
| Background | This gene encodes a deadenylase that functions as the catalytic subunit of the polyadenylate binding protein dependent poly(A) nuclease complex. The encoded protein is a magnesium dependent 3' to 5' exoribonuclease that is involved in the degradation of cytoplasmic mRNAs. Alternate splicing results in multiple transcript variants. |
| Function | Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenylation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation. |
| Cellular Localization | Cytoplasm, Nucleus |
| Calculated MW | 135 kDa |
| PTM | Phosphoprotein |
Images (3) Click the Picture to Zoom In
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ARG43886 anti-PAN2 antibody WB image
Western blot: HepG2 stained with ARG43886 anti-PAN2 antibody at 0.25 μg/mL.
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ARG43886 anti-PAN2 antibody FACS image
Flow Cytometry: PC-3 cells stained with ARG43886 anti-PAN2 antibody (blue) at 1 μg/1x10^6 cells dilution.
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ARG43886 anti-PAN2 antibody WB image
Western blot: NIH 3T3 stained with ARG43886 anti-PAN2 antibody at 0.25 μg/mL.
