ARG64016
anti-PAK1 antibody
anti-PAK1 antibody for Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes PAK1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Dog |
| Tested Application | WB |
| Specificity | No cross-reactivity exptected with PAK3 |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PAK1 |
| Antigen Species | Human |
| Immunogen | C-NTEKQKKKPKMSDE |
| Conjugation | Un-conjugated |
| Alternate Names | PAKalpha; Serine/threonine-protein kinase PAK 1; Alpha-PAK; p65-PAK; EC 2.7.11.1; PAK-1; p21-activated kinase 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P35465 Rat Serine/threonine-protein kinase PAK 1 Swiss-port # Q13153 Human Serine/threonine-protein kinase PAK 1 |
|---|---|
| Background | This gene encodes a family member of serine/threonine p21-activating kinases, known as PAK proteins. These proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling, and they serve as targets for the small GTP binding proteins Cdc42 and Rac. This specific family member regulates cell motility and morphology. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 61 kDa |
| PTM | Autophosphorylated in trans, meaning that in a dimer, one kinase molecule phosphorylates the other one. Activated by autophosphorylation at Thr-423 in response to a conformation change, triggered by interaction with GTP-bound CDC42 or RAC1. Activated by phosphorylation at Thr-423 by BRSK2 and by PDPK1. Phosphorylated by JAK2 in response to PRL; this increases PAK1 kinase activity. Phosphorylated at Ser-21 by PKB/AKT; this reduces interaction with NCK1 and association with focal adhesion sites. |
Images (2) Click the Picture to Zoom In
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ARG64016 anti-PAK1 antibody WB image
Western Blot: Rat Brain lysate (35 µg protein in RIPA buffer) stained with ARG64016 anti-PAK1 antibody at 1 µg/ml dilution.
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ARG64016 anti-PAK1 antibody WB image
Western blot: 35 µg of Human brain (cerebral cortex) lysate (in RIPA buffer) stained with ARG64016 anti-PAK1 antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
