ARG66125
anti-PAI1 / SERPINE1 antibody (Biotin)
anti-PAI1 / SERPINE1 antibody (Biotin) for ELISA and Human
Overview
| Product Description | Biotin-conjugated Rabbit Polyclonal antibody recognizes PAI1 / SERPINE1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PAI1 / SERPINE1 |
| Antigen Species | Human |
| Immunogen | E. coli derived recombinant Human PAI1 / SERPINE1. (VHHPPSYVAH LASDFGVRVF QQVAQASKDR NVVFSPYGVA SVLAMLQLTT GGETQQQIQA AMGFKIDDKG MAPALRHLYK ELMGPWNKDE ISTTDAIFVQ RDLKLVQGFM PHFFRLFRST VKQVDFSEVE RARFIINDWV KTHTKGMISN LLGKGAVDQL TRLVLVNALY FNGQWKTPFP DSSTHRRLFH KSDGSTVSVP MMAQTNKFNY TEFTTPDGHY YDILELPYHG DTLSMFIAAP YEKEVPLSAL TNILSAQLIS HWKGNMTRLP RLLVLPKFSL ETEVDLRKPL ENLGMTDMFR QFQADFTSLS DQEPLHVAQA LQKVKIEVNE SGTVASSSTA VIVSARMAPE EIIMDRPFLF VVRHNPTGTV LFMGQVMEP) |
| Conjugation | Biotin |
| Alternate Names | Serpin E1; PLANH1; Endothelial plasminogen activator inhibitor; Plasminogen activator inhibitor 1; PAI; PAI1; PAI-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by affinity chromatography. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SERPINE1 |
| Gene Full Name | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 |
| Background | This gene encodes a member of the serine proteinase inhibitor (serpin) superfamily. This member is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), and hence is an inhibitor of fibrinolysis. Defects in this gene are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1 deficiency), and high concentrations of the gene product are associated with thrombophilia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009] |
| Function | Serine protease inhibitor. This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, protein C and matriptase-3/TMPRSS7. Its rapid interaction with PLAT may function as a major control point in the regulation of fibrinolysis. [UniProt] |
| Calculated MW | 45 kDa |
| PTM | Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg-|-Met-370 bond. |
Images (2) Click the Picture to Zoom In
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ARG66125 anti-PAI1 / SERPINE1 antibody (Biotin) standard curve image
Direct ELISA: ARG66125 anti-PAI1 / SERPINE1 antibody (Biotin) at 0.25 - 1.0 µg/ml results of a typical standard run with optical density reading at 405 - 650 nm.
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ARG66125 anti-PAI1 / SERPINE1 antibody (Biotin) standard curve image
Sandwich ELISA: ARG66125 anti-PAI1 / SERPINE1 antibody (Biotin) as a detection antibody at 0.25 - 1.0 µg/ml combined with ARG66124 anti-PAI1 / SERPINE1 antibody as a capture antibody. Results of a typical standard run with optical density reading at 405 - 650 nm.
