ARG63083
anti-PAG / Cbp antibody [PAG-C1]
anti-PAG / Cbp antibody [PAG-C1] for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Bovine
Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [PAG-C1] recognizes PAG / Cbp |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov |
| Tested Application | IHC-P, IP, WB |
| Specificity | The clone PAG-C1 recognizes an epitope located in the C-terminal domain of Csk-binding protein (Cbp / PAG), a 46 kDa ubiquitously expressed transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | PAG-C1 |
| Isotype | IgG2b |
| Target Name | PAG / Cbp |
| Antigen Species | Human |
| Immunogen | C-terminal peptide (last 15 amino acids) of human Csk binding protein coupled to KLH. |
| Conjugation | Un-conjugated |
| Alternate Names | Transmembrane phosphoprotein Cbp; Csk-binding protein; CBP; PAG; Transmembrane adapter protein PAG; Phosphoprotein associated with glycosphingolipid-enriched microdomains 1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Heat-mediated antigen retrieval in citrate buffer pH 6.1 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IP: Raji IHC-P: Appendix (germinal center of lymphatic follicle) |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | PAG1 |
| Gene Full Name | phosphoprotein membrane anchor with glycosphingolipid microdomains 1 |
| Background | PAG (phosphoprotein associated with GEMs), also known as Cbp (Csk-binding protein), is a ubiquitously expressed 46 kDa transmembrane adaptor protein present in membrane rafts (glycosphingolipid-enriched microdomains), which however migrates on SDS PAGE gels anomalously as an 80 kDa molecule. Following tyrosine phosphorylation by Src family kinases, PAG binds and thereby activates the protein tyrosine kinase Csk, the major negative regulator of the Src family kinases. Signaling via the B-cell receptor in B cells or high affinity IgE receptor (FcepsilonRI) in mast cells leads to PAG increased tyrosine phosphorylation and Csk binding, while T cell receptor signaling causes PAG dephosphorylation, loss of Csk binding and increased activation of the protein tyrosine kinase Lck. |
| Function | Negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Promotes CSK activation and recruitment to lipid rafts, which results in LCK inhibition. Inhibits immunological synapse formation by preventing dynamic arrangement of lipid raft proteins. May be involved in cell adhesion signaling. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 47 kDa |
| PTM | Palmitoylated. Phosphorylated by FYN on Tyr-317 in resting T-cells; which promotes interaction with CSK. Dephosphorylated by PTPRC/CD45 upon TCR activation; which leads to CSK dissociation. May also be dephosphorylated by PTPN11. Hyperphosphorylated in mast cells upon FCER1 activation. Phosphorylated by LYN. |
Images (2) Click the Picture to Zoom In
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ARG63083 anti-PAG / Cbp antibody [PAG-C1] IP image
Immunoprecipitation: Raji lysate was immunoprecipitated with ARG63083 anti-PAG / Cbp antibody [PAG-C1] (Lane 1, 2) or anti-PAG polyclonal antibody (Lane 3). Western blot was immunostained with ARG63084 anti-PAG / Cbp antibody [MEM-255].
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ARG63083 anti-PAG / Cbp antibody [PAG-C1] IHC-P image
Immunohistochemistry: Appendix tissue section stained with ARG63083 anti-PAG / Cbp antibody [PAG-C1].
Clone References
Combined spatial and enzymatic regulation of Csk by cAMP and protein kinase a inhibits T cell receptor signaling.
Constitutive exclusion of Csk from Hck-positive membrane microdomains permits Src kinase-dependent proliferation of Theileria-transformed B lymphocytes.
