ARG59235
anti-Otoferlin antibody [13A9]
anti-Otoferlin antibody [13A9] for ICC/IF and Human
Overview
| Product Description | Mouse Monoclonal antibody [13A9] recognizes Otoferlin |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 13A9 |
| Isotype | IgG1 |
| Target Name | Otoferlin |
| Antigen Species | Human |
| Immunogen | GST fusion protein corresponding to aa. 1-395 of Human Otoferlin (Swiss-Prot: Q9HC10). |
| Conjugation | Un-conjugated |
| Alternate Names | FER1L2; DFNB6; AUNB1; DFNB9; Fer-1-like protein 2; NSRD9; Otoferlin |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Observed Size | ~ 220 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | 0.1M Tris, 0.1M Glycine and 2% Sucrose. |
| Stabilizer | 2% Sucrose |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | OTOF |
| Gene Full Name | otoferlin |
| Background | Mutations in this gene are a cause of neurosensory nonsyndromic recessive deafness, DFNB9. The short form of the encoded protein has 3 C2 domains, a single carboxy-terminal transmembrane domain found also in the C. elegans spermatogenesis factor FER-1 and human dysferlin, while the long form has 6 C2 domains. The homology suggests that this protein may be involved in vesicle membrane fusion. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion and in the control of neurotransmitter release at these output synapses. Interacts in a calcium-dependent manner to the presynaptic SNARE proteins at ribbon synapses of cochlear inner hair cells (IHCs) to trigger exocytosis of neurotransmitter. Also essential to synaptic exocytosis in immature outer hair cells (OHCs). May also play a role within the recycling of endosomes (By similarity). [UniProt] |
| Cellular Localization | Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Single-pass type II membrane protein. Basolateral cell membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. [UniProt] |
| Calculated MW | 227 kDa |
Images (1) Click the Picture to Zoom In
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ARG59235 anti-Otoferlin antibody [13A9] ICC/IF image
Immunofluorescence: Hair cells of the inner ear crista ampullaris (CA), utricular macula (UM) and saccularmacula (SM) stained with ARG59235 anti-Otoferlin antibody [13A9].
