ARG64260
anti-Ogg1 antibody
anti-Ogg1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Cancer antibody; Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Ogg1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Predict Reactivity | Ms |
| Tested Application | IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Ogg1 |
| Antigen Species | Mouse |
| Immunogen | C-HPKTSQAKGPSPLANK |
| Conjugation | Un-conjugated |
| Alternate Names | EC 4.2.99.18; N-glycosylase/DNA lyase [Includes: 8-oxoguanine DNA glycosylase; HOGG1; MUTM; OGH1; AP lyase; HMMH; apurinic or apyrimidinic site; EC 3.2.2.- |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Ogg1 |
| Gene Full Name | 8-oxoguanine DNA-glycosylase 1 |
| Background | This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008] |
| Function | DNA repair enzyme that incises DNA at 8-oxoG residues. Excises 7,8-dihydro-8-oxoguanine and 2,6-diamino-4-hydroxy-5-N-methylformamidopyrimidine (FAPY) from damaged DNA. Has a beta-lyase activity that nicks DNA 3' to the lesion. [UniProt] |
| Research Area | Cancer antibody; Gene Regulation antibody |
| Calculated MW | 39 kDa |
Images (2) Click the Picture to Zoom In
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ARG64260 anti-Ogg1 antibody WB image
Western Blot: Rat Spleen lysate (35 µg protein in RIPA buffer) stained with ARG64260 anti-Ogg1 antibody at 0.1 µg/ml dilution.
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ARG64260 anti-Ogg1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human kidney tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG64260 anti-Ogg1 antibody at 5 µg/ml dilution followed by AP-staining.
