ARG64598

anti-OTUB1 antibody

anti-OTUB1 antibody for Western blot and Mouse

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes OTUB1
Tested Reactivity Ms
Predict Reactivity Hu, Rat, Cow
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name OTUB1
Antigen Species Human
Immunogen C-YKEYAEDDNIYQQK
Conjugation Un-conjugated
Alternate Names HSPC263; hOTU1; Ubiquitin-specific-processing protease OTUB1; Deubiquitinating enzyme OTUB1; OTB1; OTU1; Otubain-1; OTU domain-containing ubiquitin aldehyde-binding protein 1; Ubiquitin thioesterase OTUB1; EC 3.4.19.12

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.03 - 0.1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 107260 Mouse OTUB1

Swiss-port # Q7TQI3 Mouse Ubiquitin thioesterase OTUB1

Background The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 31 kDa

Images (1) Click the Picture to Zoom In

  • ARG64598 anti-OTUB1 antibody WB image

    Western Blot: Mouse Brain lysate (35 µg protein in RIPA buffer) stained with ARG64598 anti-OTUB1 antibody at 0.05 µg/ml dilution.