ARG63133
anti-ORC4L antibody
anti-ORC4L antibody for Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes ORC4L |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | WB |
| Specificity | Reported variants NP_002543.2 and NP_859525.1 represent identical protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ORC4L |
| Antigen Species | Human |
| Immunogen | C-DVRQWATSSLSWL |
| Conjugation | Un-conjugated |
| Alternate Names | ORC4L; Origin recognition complex subunit 4; ORC4P |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43929 Human Origin recognition complex subunit 4 |
|---|---|
| Background | The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. This gene encodes a subunit of the ORC complex. Several alternatively spliced transcript variants, some of which encode the same protein, have been reported for this gene. [provided by RefSeq, Oct 2010] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 50 kDa |
Images (2) Click the Picture to Zoom In
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ARG63133 anti-ORC4L antibody WB image
Western Blot: Rat Heart lysate (35 µg protein in RIPA buffer) stained with ARG63133 anti-ORC4L antibody at 0.5 µg/ml dilution.
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ARG63133 anti-ORC4L antibody WB image
Western blot: 35 µg of Jurkat nuclear lysate (in RIPA buffer) stained with ARG63133 anti-ORC4L antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
