ARG62573
anti-ORC1 antibody [7F6/1]
anti-ORC1 antibody [7F6/1] for Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [7F6/1] recognizes ORC1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7F6/1 |
| Isotype | IgG1 |
| Target Name | ORC1 |
| Antigen Species | Human |
| Immunogen | Full length protein (Human) |
| Conjugation | Un-conjugated |
| Alternate Names | ORC1L; Replication control protein 1; PARC1; Origin recognition complex subunit 1; HSORC1 |
Application Instructions
| Application Note | WB: 1/1250 - 1/2500 * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | IgG purified |
| Buffer | 1X PBS buffer with <0.1% sodium azide. |
| Preservative | <0.1% sodium azide. |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13415 Human Origin recognition complex subunit 1 |
|---|---|
| Gene Symbol | ORC1 |
| Gene Full Name | origin recognition complex, subunit 1 |
| Background | The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is the largest subunit of the ORC complex. While other ORC subunits are stable throughout the cell cycle, the levels of this protein vary during the cell cycle, which has been shown to be controlled by ubiquitin-mediated proteolysis after initiation of DNA replication. This protein is found to be selectively phosphorylated during mitosis. It is also reported to interact with MYST histone acetyltransferase 2 (MyST2/HBO1), a protein involved in control of transcription silencing. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010] |
| Function | Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. [UniProt] |
| Cellular Localization | Nucleus |
| Research Area | Gene Regulation antibody |
| Calculated MW | 97 kDa |
| PTM | Phosphorylated during mitosis. |
Clone References
The role of PR-Set7 in replication licensing depends on Suv4-20h.
WB, ChIP / Human
HOXD13 binds DNA replication origins to promote origin licensing and is inhibited by geminin.
ChIP / Human, Mouse
