ARG58967

anti-OGN / Osteoglycin antibody

anti-OGN / Osteoglycin antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes OGN / Osteoglycin
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name OGN / Osteoglycin
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 20-298 of Human OGN / Osteoglycin (NP_148935.1).
Conjugation Un-conjugated
Alternate Names Mimecan; SLRR3A; OG; OIF; Osteoglycin; Osteoinductive factor

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control NIH/3T3
Observed Size 36 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18295 Mouse OGN

GeneID: 4969 Human OGN

Swiss-port # P20774 Human Mimecan

Swiss-port # Q62000 Mouse Mimecan

Gene Symbol OGN
Gene Full Name osteoglycin
Background This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR). Alternatively spliced transcript variants that encode the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Induces bone formation in conjunction with TGF-beta-1 or TGF-beta-2. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Calculated MW 34 kDa
PTM N- and O-glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG58967 anti-OGN / Osteoglycin antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG58967 anti-OGN / Osteoglycin antibody at 1:100 dilution.

  • ARG58967 anti-OGN / Osteoglycin antibody WB image

    Western blot: 25 µg of NIH/3T3 cell lysate stained with ARG58967 anti-OGN / Osteoglycin antibody at 1:1000 dilution.