ARG22637
anti-O-GlcNAc antibody [9H6]
anti-O-GlcNAc antibody [9H6] for ICC/IF,Western blot and Other
Overview
| Product Description | Mouse Monoclonal antibody [9H6] recognizes O-GlcNAc |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ICC/IF, WB |
| Specificity | Specific for β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with N-Acetylgalactosamine, Glucose, or Galactose modified proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 9H6 |
| Isotype | IgM |
| Target Name | O-GlcNAc |
| Antigen Species | Others |
| Immunogen | Synthetic β-O-Linked N-Acetylglucosamine conjugated to KLH. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | PEG Purified. |
| Buffer | PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol. |
| Preservative | 0.9% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (2) Click the Picture to Zoom In
-
ARG22637 anti-O-GlcNAc antibody [9H6] ICC/IF image
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22637 anti-O-GlcNAc antibody [9H6] at 1:50 for 30 - 60 min at RT. Secondary Antibody: Goat anti-Mouse (Alexa Fluor 488) at 1:1500 for 30 - 60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 F-Actin stain. (C) Primary antibody (D) Composite. .
-
ARG22637 anti-O-GlcNAc antibody [9H6] WB image
Western blot: 2.0 µg of BSA, GlcNAc-BSA, GalNAc-BSA, Galactose-BSA, Glucose-BSA, and Citrulline-BSA stained with ARG22637 anti-O-GlcNAc antibody [9H6] at 1:1000 for 2 hours at RT.
