ARG22636
anti-O-GalNAc + O-GlcNAc antibody [9B9]
anti-O-GalNAc + O-GlcNAc antibody [9B9] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Other
Overview
| Product Description | Mouse Monoclonal antibody [9B9] recognizes O-GalNAc + O-GlcNAc |
|---|---|
| Tested Reactivity | Other |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Specific for β-O-Linked N-Acetylgalactosamine and β-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 9B9 |
| Isotype | IgM |
| Target Name | O-GalNAc + O-GlcNAc |
| Antigen Species | Others |
| Immunogen | Synthetic β-O-Linked N-Acetylglucosamine conjugated to KLH. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | PEG Purified. |
| Buffer | PBS (pH 7.4), 0.9% Sodium azide and 50% Glycerol. |
| Preservative | 0.9% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Images (3) Click the Picture to Zoom In
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ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] ICC/IF image
Immunofluorescence: 5% Formaldehyde fixed (5 min) HEK293 cells stained with ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] (green) at 1:50 dilution for 30 - 60 min at RT. (A) DAPI (blue) for nuclear staining. (B) Phalloidin Alex Fluor 633 (red) for F-Actin staining. (C) Primary antibody. (D) Composite.
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ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse colon tissue stained with ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] (green) at 1:25 dilution for 1 hour at RT. (A) DAPI (blue) for nuclear staining. (B) Phalloidin Alex Fluor 633 (red) for F-Actin staining. (C) Primary antibody. (D) Composite.
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ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] WB image
Western blot: 2.0 µg of GlcNAc-BSA, GalNAc-BSA, Galactose-BSA, and Glucose-BSA stained with ARG22636 anti-O-GalNAc + O-GlcNAc antibody [9B9] at 1:1000 for 2 hours at RT.
